Construct:Article Title: Efficacy and safety of autologous CD5-KO anti-CD5 CAR-T cells in relapsed/refractory CD5 + hematological malignancies.
Article Snippet: Post-isolation, T cells were activated with Dynabeads Mouse T-Activator CD3/CD28 (Gibco) at a concentration of 10 μL per 1×10 6 cells and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) (Gibco), 50 μM β-mercaptoethanol, 1% non-essential amino acids (NEAA), 1% sodium pyruvate, 1% HEPES, 1% GlutaMax, and 200 IU/mL interleukin-2 (IL-2). .. REAGENT or RESOURCE SOURCE IDENTIFIER D-luciferin sodium Solarbio Cat# D9390 DAB Horseradish Peroxidase ColorDevelopment Kit beyotime Cat# P0203) Experimental models: Cell lines Molt-4 cell line ATCC Cat# CRL-1582 SUP-T1 cell line ATCC Cat# CRL-1942 Plat-E cell line ATCC Cat# CRL-1573 Molt-4-luciferase cell line Constructed by our lab N/A SUP-T1-luciferase cell line Constructed by our lab N/A Experimental models: Organisms/strains Mouse: C57BL/6-Tg (CAG-EGFP)1Osb/J Shulaibao Biotechnology N/A Mouse: C57BL/6JGpt-Cd5em1Cin(hCD5)/Gpt GemPharmatech N/A Recombinant DNA Plasmid: mCAR Constructed by our lab N/A Software and algorithms ImageJ v1.53 National Institutes of Health (NIH) https://imagej.net/ij/ Graphpad prism 9 GraphPad Software Inc https://www.graphpad.com FlowJo V10 FlowJo https://www.flowjo.com/ R The R Project for Statistical Computing https://www.r-project.org/ Other Clinical Trial Registry ClinicalTrials.gov NCT04767308 RPMI 1640 Gibco Cat# 11875093 DPBS Gibco Cat# 14190144 PBS Biosharp Cat# BL302A Opti-MEM Gibco Cat# 31985070 Fetal Bovine Serum Gibco Cat# A5669701 DMEM Gibco Cat# 11965092 Cell Reports Medicine 7, 102584, February 17, 2026 e2 OPEN ACCESS .. GFP knock-in (C57BL/6-Tg (CAG-EGFP)1Osb/J) and hCD5 knock-in (C57BL/6JGpt-Cd5em1Cin(hCD5)/Gpt) mice were purchased from Shulaibao Biotechnology Co., Ltd. and GemPharmatech Co., Ltd, respectively.
Recombinant:Article Title: Efficacy and safety of autologous CD5-KO anti-CD5 CAR-T cells in relapsed/refractory CD5 + hematological malignancies.
Article Snippet: Post-isolation, T cells were activated with Dynabeads Mouse T-Activator CD3/CD28 (Gibco) at a concentration of 10 μL per 1×10 6 cells and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) (Gibco), 50 μM β-mercaptoethanol, 1% non-essential amino acids (NEAA), 1% sodium pyruvate, 1% HEPES, 1% GlutaMax, and 200 IU/mL interleukin-2 (IL-2). .. REAGENT or RESOURCE SOURCE IDENTIFIER D-luciferin sodium Solarbio Cat# D9390 DAB Horseradish Peroxidase ColorDevelopment Kit beyotime Cat# P0203) Experimental models: Cell lines Molt-4 cell line ATCC Cat# CRL-1582 SUP-T1 cell line ATCC Cat# CRL-1942 Plat-E cell line ATCC Cat# CRL-1573 Molt-4-luciferase cell line Constructed by our lab N/A SUP-T1-luciferase cell line Constructed by our lab N/A Experimental models: Organisms/strains Mouse: C57BL/6-Tg (CAG-EGFP)1Osb/J Shulaibao Biotechnology N/A Mouse: C57BL/6JGpt-Cd5em1Cin(hCD5)/Gpt GemPharmatech N/A Recombinant DNA Plasmid: mCAR Constructed by our lab N/A Software and algorithms ImageJ v1.53 National Institutes of Health (NIH) https://imagej.net/ij/ Graphpad prism 9 GraphPad Software Inc https://www.graphpad.com FlowJo V10 FlowJo https://www.flowjo.com/ R The R Project for Statistical Computing https://www.r-project.org/ Other Clinical Trial Registry ClinicalTrials.gov NCT04767308 RPMI 1640 Gibco Cat# 11875093 DPBS Gibco Cat# 14190144 PBS Biosharp Cat# BL302A Opti-MEM Gibco Cat# 31985070 Fetal Bovine Serum Gibco Cat# A5669701 DMEM Gibco Cat# 11965092 Cell Reports Medicine 7, 102584, February 17, 2026 e2 OPEN ACCESS .. GFP knock-in (C57BL/6-Tg (CAG-EGFP)1Osb/J) and hCD5 knock-in (C57BL/6JGpt-Cd5em1Cin(hCD5)/Gpt) mice were purchased from Shulaibao Biotechnology Co., Ltd. and GemPharmatech Co., Ltd, respectively.
Plasmid Preparation:Article Title: Efficacy and safety of autologous CD5-KO anti-CD5 CAR-T cells in relapsed/refractory CD5 + hematological malignancies.
Article Snippet: Post-isolation, T cells were activated with Dynabeads Mouse T-Activator CD3/CD28 (Gibco) at a concentration of 10 μL per 1×10 6 cells and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) (Gibco), 50 μM β-mercaptoethanol, 1% non-essential amino acids (NEAA), 1% sodium pyruvate, 1% HEPES, 1% GlutaMax, and 200 IU/mL interleukin-2 (IL-2). .. REAGENT or RESOURCE SOURCE IDENTIFIER D-luciferin sodium Solarbio Cat# D9390 DAB Horseradish Peroxidase ColorDevelopment Kit beyotime Cat# P0203) Experimental models: Cell lines Molt-4 cell line ATCC Cat# CRL-1582 SUP-T1 cell line ATCC Cat# CRL-1942 Plat-E cell line ATCC Cat# CRL-1573 Molt-4-luciferase cell line Constructed by our lab N/A SUP-T1-luciferase cell line Constructed by our lab N/A Experimental models: Organisms/strains Mouse: C57BL/6-Tg (CAG-EGFP)1Osb/J Shulaibao Biotechnology N/A Mouse: C57BL/6JGpt-Cd5em1Cin(hCD5)/Gpt GemPharmatech N/A Recombinant DNA Plasmid: mCAR Constructed by our lab N/A Software and algorithms ImageJ v1.53 National Institutes of Health (NIH) https://imagej.net/ij/ Graphpad prism 9 GraphPad Software Inc https://www.graphpad.com FlowJo V10 FlowJo https://www.flowjo.com/ R The R Project for Statistical Computing https://www.r-project.org/ Other Clinical Trial Registry ClinicalTrials.gov NCT04767308 RPMI 1640 Gibco Cat# 11875093 DPBS Gibco Cat# 14190144 PBS Biosharp Cat# BL302A Opti-MEM Gibco Cat# 31985070 Fetal Bovine Serum Gibco Cat# A5669701 DMEM Gibco Cat# 11965092 Cell Reports Medicine 7, 102584, February 17, 2026 e2 OPEN ACCESS .. GFP knock-in (C57BL/6-Tg (CAG-EGFP)1Osb/J) and hCD5 knock-in (C57BL/6JGpt-Cd5em1Cin(hCD5)/Gpt) mice were purchased from Shulaibao Biotechnology Co., Ltd. and GemPharmatech Co., Ltd, respectively.
Software:Article Title: Efficacy and safety of autologous CD5-KO anti-CD5 CAR-T cells in relapsed/refractory CD5 + hematological malignancies.
Article Snippet: Post-isolation, T cells were activated with Dynabeads Mouse T-Activator CD3/CD28 (Gibco) at a concentration of 10 μL per 1×10 6 cells and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) (Gibco), 50 μM β-mercaptoethanol, 1% non-essential amino acids (NEAA), 1% sodium pyruvate, 1% HEPES, 1% GlutaMax, and 200 IU/mL interleukin-2 (IL-2). .. REAGENT or RESOURCE SOURCE IDENTIFIER D-luciferin sodium Solarbio Cat# D9390 DAB Horseradish Peroxidase ColorDevelopment Kit beyotime Cat# P0203) Experimental models: Cell lines Molt-4 cell line ATCC Cat# CRL-1582 SUP-T1 cell line ATCC Cat# CRL-1942 Plat-E cell line ATCC Cat# CRL-1573 Molt-4-luciferase cell line Constructed by our lab N/A SUP-T1-luciferase cell line Constructed by our lab N/A Experimental models: Organisms/strains Mouse: C57BL/6-Tg (CAG-EGFP)1Osb/J Shulaibao Biotechnology N/A Mouse: C57BL/6JGpt-Cd5em1Cin(hCD5)/Gpt GemPharmatech N/A Recombinant DNA Plasmid: mCAR Constructed by our lab N/A Software and algorithms ImageJ v1.53 National Institutes of Health (NIH) https://imagej.net/ij/ Graphpad prism 9 GraphPad Software Inc https://www.graphpad.com FlowJo V10 FlowJo https://www.flowjo.com/ R The R Project for Statistical Computing https://www.r-project.org/ Other Clinical Trial Registry ClinicalTrials.gov NCT04767308 RPMI 1640 Gibco Cat# 11875093 DPBS Gibco Cat# 14190144 PBS Biosharp Cat# BL302A Opti-MEM Gibco Cat# 31985070 Fetal Bovine Serum Gibco Cat# A5669701 DMEM Gibco Cat# 11965092 Cell Reports Medicine 7, 102584, February 17, 2026 e2 OPEN ACCESS .. GFP knock-in (C57BL/6-Tg (CAG-EGFP)1Osb/J) and hCD5 knock-in (C57BL/6JGpt-Cd5em1Cin(hCD5)/Gpt) mice were purchased from Shulaibao Biotechnology Co., Ltd. and GemPharmatech Co., Ltd, respectively.
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